Gypenoside L

Research use only, not for human use.

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release.

Gypenoside L inhibits autophagic flux and induces cell death in human esophageal cancer cells through endoplasm reticulum stress-mediated Ca2+ release. It also inhibit non-small cell lung carcinoma A549 cell inhibitory activity.gypenoside L (Gyp-L), a saponin isolated from Gynostemma pentaphyllum, on cancer cell growth. We found that Gyp-L increased the SA-β-galactosidase activity, promoted the production of senescence-associated secretory cytokines, and inhibited cell proliferation of human liver and esophageal cancer cells. Moreover, Gyp-L caused cell cycle arrest at S phase, and activated senescence-related cell cycle inhibitor proteins (p21 and p27) and their upstream regulators. In addition, Gyp-L activated p38 and ERK MAPK pathways and NF-κB pathway to induce senescence. Consistently, adding chemical inhibitors efficiently counteracted the Gyp-L-mediated senescence, growth inhibition, and cell cycle arrest in cancer cells. Furthermore, treatment with Gyp-L, enhanced the cytotoxicity of clinic therapeutic drugs, including 5-fluorouracil and cisplatin, on cancer cells. Overall, these results indicate that Gyp-L inhibits proliferation of cancer cells by inducing senescence and renders cancer cells more sensitive to chemotherapy.

Gypenoside L (20-80 μg/mL; 24 h) increases the mRNA expression levels of SASP, such as IL-1α, IL-6, TIMP-1, CXCL-1 and CXCL-2 in HepG2 and ECA-109 cells.Gypenoside L (20-80 μg/mL; 24 h) causes cell cycle arrest at S phase.GPL (3.125-100 μg/mL) significantly inhibits LPS-induced NO accumulation in RAW264.7 cells.

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Immunology/Inflammation

ROS

ROS|Calcium Channel

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94987-09-4

801

C42H72O14

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (124.84 mM)

C/C(C)=C\CC[C@](C)(O)[C@H]6CC[C@]5(C)[C@@H]6[C@H](O)C[C@H]4[C@@]5(C)CC[C@H]3C(C)(C)[C@@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)[C@H](O)C[C@@]34C

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(-20℃)

With Ice Pack

≥ 2 years