NSC 19630

Research use only, not for human use.

NSC 19630 (MIRA-1) is a potent, selective Werner syndrome helicase (WRN helicase) with IC50 of 20 uM.

NSC 19630 (MIRA-1) is a potent, selective Werner syndrome helicase (WRN helicase) with IC50 of 20 uM; shows selecitvity over human RecQ helicases (RECQ1 and BLM), Fanconi anemia group J (FANCJ) helicases and three Escherichia coli helicases (RecQ, UvrD, and DnaB); dramatically impaires growth and proliferation, induces apoptosis in a WRN-dependent manner, and resultes in elevated γ-H2AX and PCNA foci in human cells; delays S-phase progression and induces DNA damage; a mutant p53 reactivator that induces mutant p53-dependent cell death in different human tumor cells carrying tetracycline-regulated mutant p53.

Cell Proliferation Assay Cell Line:Saos-2-His273, H1299-His175, SKOV-His175 and SKOV-His273 Concentration:25 μM Incubation Time:48 hours Result:Inhibited cell growth in a mutant p53-dependent manner, with survival rate of 18.5~39% in no doxycycline and 71.5~87.5% in doxycycline.Apoptosis Analysis Cell Line:Saos-2 and Saos-2-His273 cells Concentration:10 μM Incubation Time:48 hours Result:Caused a substantial increase in the fraction of cells with a sub-G1 DNA content in the presence of mutant p53.

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MIRA-1

Cell Cycle/DNA Damage

DNA Repair Protein

DNA Repair Protein

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72835-26-8

183.2

C8H9NO4

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (545.97 mM)

O=C(C=C1)N(COC(CC)=O)C1=O

1-[(1-oxopropoxy)methyl]-1H-pyrrole-2,5-dione

(-20℃)

With Ice Pack

≥ 2 years