Deoxypodophyllotoxin

Research use only, not for human use.

Deoxypodophyllotoxin shows cytotoxic , antineoplastic, antitumor, insecticidal, anti-angiogenic, vascular disrupting, insecticidal, antiviral, and anti-inflammatory activities.Deoxypodophyllotoxin (DPT) induced both apoptosis and autophagy via production of mitochondrial reactive oxygen species (ROS).

Deoxypodophyllotoxin shows cytotoxic , antineoplastic, antitumor, insecticidal, anti-angiogenic, vascular disrupting, insecticidal, antiviral, and anti-inflammatory activities.Deoxypodophyllotoxin (DPT) induced both apoptosis and autophagy via production of mitochondrial reactive oxygen species (ROS). DPT suppressed the PI3K/AKT/mTOR signaling cascades to lead autophagy process, resulting from conversion of light chain 3-I (LC3-I) into LC3-II and acidic vesicular organelles (AVOs) formation. Even if DPT-induced ROS were occurred in both apoptosis and autophagy, inhibition of ROS generation enhanced cell viability. Otherwise, 3-methyladeine (3-MA) impeding on autophagy accelerated an apoptotic response caused by DPT. Therefore, these findings suggest that DPT triggers cytoprotective autophagy against cytotoxic apoptosis.

Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell populationfrom 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively.Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners. Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity. Apoptosis Analysis Cell Line:SGC-7901 cells Concentration:25, 50, 75 nM Incubation Time:6, 12, 24, 48 hours Result:Induced apoptosis in SGC-7901 Cells.Cell Cycle AnalysisCell Line:SGC-7901 cells Concentration:25, 50, 75 nM Incubation Time:6, 12, 24, 48 hours Result:Induced G2/M cell cycle arrest in SGC-7901 Cells Western Blot Analysis Cell Line:SGC-7901 cells Concentration:25, 50, 75 nM Incubation Time:6, 12, 24, 48 hoursResult:Altered the expression of cyclin B1, Cdc2,Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins.

Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively. Animal Model:Xenograft model of gastric cancer in nude mice with SGC-7901 cells Dosage:5, 10, and 20 mg/kg Administration:Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days Result:Inhibited the growth of gastric cancer tumors.

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Cytoskeleton/Cell Adhesion Molecules

Microtubule/Tubulin

microtubule|Autophagy|Apoptosis

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19186-35-7

398.41

C22H22O7

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (251.00 mM)

[H][C@]12COC(=O)[C@]1([H])[C@H](c1cc(OC)c(OC)c(OC)c1)c1cc3OCOc3cc1C2

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(-20℃)

With Ice Pack

≥ 2 years