Ciprofloxacin hydrochloride

Research use only, not for human use.

A broad-spectrum antimicrobial carboxyfluoroquinoline.

A broad-spectrum antimicrobial carboxyfluoroquinoline.(In Vitro):Ciprofloxacin (Bay-09867) monohydrochloride (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase.Ciprofloxacin (Bay-09867) monohydrochloride shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively.(In Vivo):Ciprofloxacin (Bay-09867) monohydrochloride (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague.Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall.Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall.

Ciprofloxacin (Bay-09867) monohydrochloride (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase.Ciprofloxacin (Bay-09867) monohydrochloride shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively. Cell Viability Assay Cell Line:Tendon cells Concentration:5, 10, 20 and 50 μg/mL Incubation Time:24 hours Result:Decreased the cellularity of tendon cells.Cell Cycle Analysis Cell Line:Tendon cells Concentration:50 μg/mL Incubation Time:24 hours Result:Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells.Western Blot Analysis Cell Line:Tendon cells Concentration:50 μg/mL Incubation Time:0, 6, 12, 17 and 24 hours Result:Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulatedthe expression of PLK-1 protein.

Ciprofloxacin (Bay-09867) monohydrochloride (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague.Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall. Ciprofloxacin (Bay-09867) monohydrochloride (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall. Animal Model:BALB/c mice Dosage:30 mg/kg Administration:Intraperitoneal injection; for 24 hours Result:Reduced the lung bacterial load in murine model of pneumonic plague.Animal Model:C57BL/6J mice Dosage:100 mg/kg Administration:Oral gavage; daily, for 4 weeks Result:Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity.Animal Model:C57BL/6J mice Dosage:100 mg/kg Administration:Oral gavage; daily, for 4 weeks Result:Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.

BAY-o 9867 | NSC 620634

Cell Cycle/DNA Damage

DNA gyrase

DNA gyrase| Topo II| Topo IV

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93107-08-5

367.8

C17H18FN3O3·HCl

>98% (HPLC)

DMSO: 10 mM

Cl.OC(=O)C1=CN(C2CC2)C2=CC(N3CCNCC3)=C(F)C=C2C1=O

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(-20℃)

With Ice Pack

≥ 2 years