Tebuconazole

Research use only, not for human use.

Tebuconazole is an agricultural azole fungicide.

Tebuconazole is an agricultural azole fungicide. It can also inhibit CYP51 (IC50s: 0.9 and 1.3 μM for Candida albicans CYP51 and truncated Homo sapiens CYP51, respectively).

Western Blot Analysis Cell Line:HepG2 cells Concentration:20,40,80 μM Incubation Time:1–12 hours Result:Increased the nuclear translocation of peroxisome proliferator-activated receptors and the expression of cluster of differentiation 36, fatty acid transport protein (FATP) 2, FATP5, and carnitine palmitoyltransferase 1. Apoptosis AnalysisCell Line:Bovine mammary gland epithelial cells (MAC-T cells Concentration: 100,150,200,250,500,750 μM Incubation Time:24 hours Result:Decreased cells viability and proliferation and activates apoptotic cell death via the upregulation of pro-apoptotic proteins, such as cleaved caspases 3 and 8 and BAX.Induced loss of mitochondrial membrane potential in MAC-T cells.Induced mitochondria-mediated apoptotic MAC-T cell death by activating ER stress.Induced endoplasmic reticulum (ER) stress via the upregulation of Bip/GRP78; PDI; ATF4; CHOP; and ERO1-Lα.

Animal Model:Male Wistar ratsDosage:10, 25, and 50 mg/kg Administration:p. o. once daily for 28 days Result:Induced CYP1A1/2, CYP2B1/2, CYP2E1, and CYP3A proteins in liver.Decreased glutathione content and increased glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase activities in liver . Increased superoxide dismutase activities in kidney and testis.Decreased glutathione S-transferase activity in testis .Decreased serum testosterone concentration and cauda epididymal sperm count .Animal Model:Male and female Sprague-Dawley ratsDosage:25, 50, and 100 mg/kg Administration:Oral gavage (p.o.), for 10 days Result:Increased fetal serum testosterone and progesterone levels.Increased the number of fetal Leydig cells per testis without inducing cell aggregation. Up-regulated the expression levels of Star, Cyp11a1, Hsd17b3, and Fshr.Increased phosphorylation of AKT1, ERK1/2, and mTOR, the level of BCL2, as well as the decrease of Beclin1, LC3B, and BAX.

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Metabolic Enzyme/Protease

P450

CYP51

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107534-96-3

307.82

C16H22ClN3O

>98% (HPLC)

DMSO:50 mg/mL (162.43 mM)

CC(C)(C)C(CCc(cc1)ccc1Cl)(Cn1ncnc1)O

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(-20℃)

With Ice Pack

≥ 2 years