Girinimbine

Research use only, not for human use.

Girinimbine (Girinimbin) is a carbazole alkaloid isolated from the plants M. koenigii, M. koenigii, and Murraya koenigii.

Girinimbine (Girinimbin) is a carbazole alkaloid with a variety of biological effects. Girinimbine can induce apoptosis, and has antitrypanosomal, antiplatelet activity, antibacterial activity, anti-inflammatory, antioxidant and antitumor activities.

Girinimbine (1-400 μM; 24-72 h) decreases the viability of HepG2 cells in 24, 48 and 72 h with IC50 values of 61 μM, 56 μM, and 40 μM respectively. Girinimbine (10-100 μM; 24-48 h) increase of LDH leakage in both concentration- and time-dependent manner in HepG2 cells.Girinimbine (56 μM; 24-48 h) treatment results in DNA fragmentation and elevates levels of caspase-3 in HepG2 cells.HepG2 cells.Girinimbine (56 μM; 12-48 h) treatment also displays a time-dependent accumulation of the Sub-G0/G1 peak (hypodiploid) and caused G0/G1-phase arrest.Girinimbine shows a potent antitrypanosomal activitywith an IC50 value of 10.16 μg/mL.Cell Viability Assay Cell Line:HepG2 cells Concentration:1 μM, 10 μM, 50 μM, 100 μM, 200 μM Incubation Time:24 h, 48 h and 72 h Result:Inhibited the proliferation of HepG2 cells in vitro in a dose- and time-dependent manner.Apoptosis Analysis Cell Line:HepG2 cells Concentration:56 μM Incubation Time:24 h, 48 h Result:Showed typical morphological features of apoptosis.Cell Cycle Analysis Cell Line:HepG2 cells Concentration:56 μM Incubation Time:12 h, 24 h, 48 h Result:Induced G0/G1-phase arrest in HepG2 cells.

Girinimbine (10-100 mg/kg; orally gavage; once) pretreatment helps limit total leukocyte migration, and reduced pro-inflammatory cytokine (IL-1β, TNF-α) levels in the peritoneal fluid. In vivo in zebrafish embryos, Girinimbine (20 μg/mL; 24 hours) shows significant distribution of apoptotic cells in embryos.Animal Model:Male ICR mice (25-35 g) treated with carrageenan Dosage:10 mg/kg, 30 mg/kg, and 100 mg/kg Administration: Orally gavage; once Result:Helped limit total leukocyte migration, and reduced pro-inflammatory cytokine levels in the peritoneal fluid.

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Others

Antioxidant

Antioxidant | Apoptosis | Antibacterial

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23095-44-5

263.33

C18H17NO

>98% (HPLC)

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Cc1cc2c3ccccc3[nH]c2c2C=CC(C)(C)Oc12

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(-20℃)

With Ice Pack

≥ 2 years