L82

Research use only, not for human use.

L82 is a DNA ligase 1 (DNA Lig1) inhibitor (hLig1 IC50=12 μM) that is selective and non-competitive.L82 expresses anti-proliferative activity against breast cancer cells.

L82 is a selective and uncompetitive DNA ligase 1 (DNA Lig1) inhibitor (hLig1 IC50=12 μM). L82 shows anti-proliferative activity to breast cancer cells.

L82 (0-50 μM; 6 d) shows anti-proliferative activity to breast cancer cells.L82 (50 μM; 0-48 h) shows cytostatic activity due to activation of the G1/S checkpoint in MCF7 cells.Cell Proliferation Assay Cell Line:MCF10A, MCF7, HCT116, and HeLa cells Concentration:0-50 μM Incubation Time:6 days Result:Reduced the proliferation of a normal breast epithelial cell line MCF10A and the breast cancer cell lines MCF7, HeLa and HCT116, in a concentration-dependent manner.Cell Cycle Analysis Cell Line:MCF7 cells Concentration:50 μM Incubation Time:0-48 hours Result:Showed a transient accumulation of cells at G2/M after 12 h, then showed an accumulation at G0/G1 that peaked after 24 h.Decreased in the S phase cell in accompany with the increase in the G0/G1 phase.

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Cell Cycle/DNA Damage

DNA/RNA Synthesis

DNA

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329227-30-7

309.67

C11H8ClN5O4

>98% (HPLC)

In Vitro:?DMSO : 33.33 mg/mL (107.63 mM; Ultrasonic )

Oc1ccc(\C=N\Nc2cn[nH]c(=O)c2Cl)cc1[N+]([O-])=O

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(-20℃)

With Ice Pack

≥ 2 years