YS-49

Research use only, not for human use.

YS-49 is an activator of PI3K/Akt (a downstream target of RhoA).

YS-49 is an activator of PI3K/Akt (a downstream target of RhoA).(In Vitro):In RAVSMC and RAW 264.7 cells, YS-49 (1-100 μM; 18 hours; ) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC50 values of 22 μM and 30 μM, respectively.At the transcriptional level, YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells .(In Vivo):In male Sprague Dawley rats,YS-49 (5 mg/kg; intraperitoneal injection; 8 hours) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM.

YS-49 (1-100?μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC50 values of 22 μM and 30?μM, respectively.YS-49 (10-100?μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level. Cell Viability Assay Cell Line:RAVSMC and RAW 264.7 cells Concentration:10 μΜ, 30 μΜ and 100?μΜ (RAVSMC cells); 1 μΜ, 10 μΜ and 100?μM (RAW 264.7 cells)Incubation Time:18 hours Result:Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC50 values of 22 and 30?μM, respectively. Western Blot Analysis Cell Line:RAVSMC and RAW 264.7 cells Concentration:10 μΜ, 30 μΜ and 100?μΜ Incubation Time:18 hours Result:Concentration-dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ.

YS-49 (5?mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM. Animal Model:Male Sprague Dawley rats (250-300?g)Dosage:5?mg/kg Administration:Intraperitoneal injection; 8 hours Result:Serum NOx levels were significantly reduced.

——

Endocrinology/Hormones

RAAS

APE1| Parasite| Topoisomerase I| Topoisomerase II

——

——

132836-42-1

386.289

C20H20BrNO2

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (258.88 mM)

Br.Oc1cc2CCNC(Cc3cccc4ccccc34)c2cc1O

——

(-20℃)

With Ice Pack

≥ 2 years