Poly(I:C)

Research use only, not for human use.

Poly (I:C) is a synthetic double-stranded RNA (dsRNA) analog and is an immunostimulant that acts as the most potent interferon (IFN) inducer. It also is a Toll-like receptor- 3 (TLR3) agonist.Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the poly (I:C) can produce 3-d mature dendritic cells.

Poly (I:C) is a synthetic double-stranded RNA (dsRNA) analog and is an immunostimulant that acts as the most potent interferon (IFN) inducer. It also is a Toll-like receptor- 3 (TLR3) agonist.Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the poly (I:C) can produce 3-d mature dendritic cells .

Cell Cytotoxicity Assay Cell Line:16HBE14o- cells Concentration:5 μg/mL Incubation Time:24 h Result:Did not lead to significant accumulation of LDH in cell-culture medium

Animal Model:lung tumor-bearing mice Dosage:10 μg/mouse Administration:Intraperitoneal injection (i.p.)Result:Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.Reduced the amount of lung foci to ≈ 40%.Significantly increased BAL fluid cell numbers. Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.Increased TLR3 expression.Animal Model:Middle cerebral artery occlusion (MCAO) model mice Dosage:1.25 mg/kg Administration:Intraperitoneal injection (i.p.)Result:Reduced focal cerebral I/R injury.Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.

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Immunology/Inflammation

TLR

TLR3

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24939-03-5

671.41

C19H27N7O16P2

>98% (HPLC)

H2O:21.5mg/mL;DMSO:<1 mg/mL

O[C@@H]([C@H](N1C(NC=NC2=O)=C2N=C1)O[C@@H]3COP(O)(O)=O)[C@@H]3O.O[C@H]([C@@H](COP(O)(O)=O)O[C@H]4N5C=CC(N)=NC5=O)[C@@H]4O

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(-20℃)

With Ice Pack

≥ 2 years