Cardiogenol C hydrochloride(b)

Research use only, not for human use.

Cardiogenol C hydrochloride can induces the differentiation of ESCs into cardiomyocytes (EC50= 100 nM).

Cardiogenol C hydrochloride can induces the differentiation of ESCs into cardiomyocytes (EC50= 100 nM).

Cardiogenol C hydrochloride (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF,?nppa) in P19 cells when it compares to untreated control cells.Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells.Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells.Cardiogenol C hydrochloride (1 μM; 35 days) addition from day 0 significantly increases myocardial differentiation and results in a significantly increased percentage of CBs with beating cardiomyocytes. This small moleculepromotes the development of beating cardiomyocytes in cardiovascular progenitor cell-derived cardiac bodies.Cardiogenol C hydrochloride (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells. RT-PCR Cell Line:C2C12 cells Concentration:0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM Incubation Time:7 days Result:Increased ANF and NKX2.5 mRNA level as a dose-dependent manner. Western Blot Analysis Cell Line:C2C12 cells Concentration:0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM Incubation Time:7 days Result:Increased cardiac Nav1.5 sodium channel protein levels.Cell Proliferation Assay Cell Line:C2C12 cells Concentration:0.01 μM; 0.1 μM; 1 μM Incubation Time:7 days Result:Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.

——

Cardiogenol C | Cardiogenol C HCl

Wnt/Notch/Hedgehog

Wnt/beta/catenin

Wnt

——

——

1049741-55-0

296.75

C13H17ClN4O2

>98% (HPLC)

DMSO:59 mg/mL (198.82 mM)H2O:2 mg/mL (6.74 mM; Need ultrasonic)

COc1ccc(Nc2nccc(NCCO)n2)cc1.Cl

——

(-20℃)

With Ice Pack

≥ 2 years