Agrimol B

Research use only, not for human use.

Agrimol B is a main active ingredient isolated from Agrimonia pilosa Ledeb.

Agrimol B is a main active ingredient isolated from Agrimonia pilosa Ledeb.

Cell Cycle Analysis Cell Line:PC-3 and A549 cells Concentration:29 and 63 μM (PC-3), 19 and 76 μM (A549)Incubation Time:10 min Result:Dose-dependently increased cells at G0 in both cell lines. Reduced percentage of cells positive for Ki-67, and increased p27 positive cell population in PC-3 and A549 cells.Western Blot Analysis Cell Line:PC-3 and A549 cells Concentration:0, 29, 49, 63 μM (PC-3); 0, 19, 50, and 76 μM (A549) Incubation Time:3 days Result:Reduced c-MYC, SKP2 and increased p27 in both cell lines, and down-regulated SPT16 and SSRP1 in A549 cells with no effect on CRM1 in both cell lines.Immunofluorescence Cell Line:3T3-L1 preadipocyte Concentration:0, 3, and 10 μM Incubation Time:6 days Result:Significantly increased nuclear positive rate of SIRT1; Markedly increased SIRT1 expression at 10 μM, the effect vanished at 3 μM. Down-regulated PPARγ and C/EBPα (CCAAT/enhancer-binding protein α) expression; Significantly decreased FAS (fatty acid synthesis), UCP-1 (uncoupling protein-1), and apoE (apolipoprotein E) expression at 10 μM.

Animal Model:BALB/c nude mice (6-week-old, male, injected with PC-3 cells in 0.2 mL PBS subcutaneously)Dosage:10 mg/kg Administration:Orally, daily, after 15 days of cancer cell implantation Result:Inhibited tumor growth in a mouse model of human prostate cancer, reduced the tumor volume at day 31 and day 32.

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Apoptosis

NF-κB

Others

Others-Field

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55576-66-4

682.76

C37H46O12

>98% (HPLC)

In Vitro:?DMSO : 5.56 mg/mL (8.14 mM)

CCCC(=O)C1=C(C(=C(C(=C1O)CC2=C(C(=C(C(=C2O)C(=O)C(C)CC)O)CC3=C(C(=C(C(=C3O)C)OC)C(=O)CCC)O)O)O)C)OC

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(-20℃)

With Ice Pack

≥ 2 years