Timosaponin AIII

Research use only, not for human use.

Timosaponin AIII induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM).

Timosaponin AIII induces autophagy in HeLa cells followed by apoptotic cell death (IC50: 10 μM). The Timosaponin AIII cellular response is mediated via inhibition of mTORC1 and induction of ER stress (IC50: 2.5 μM, BT474 cells; 6 μM, MDAMB231). The Timosaponin AIII pro-apoptotic response is selective for tumor cells over normal cells but autophagy is induced in both.

Timosaponin AIII could inhibit acetylcholinesterase (AChE) activity, with an IC50 of 35.4 μM. Timosaponin AIII is identified as a major selective cytotoxic activity in BN108, and its selective cytotoxic activity involves inhibition of mTOR, induction of ER stress and protective autophagy.

Of the tested steroidal saponins, Timosaponin AIII (TA3) most potently improves memory deficits. Timosaponin AIII increases the scopolamine-induced reduction in step-through latency by 17% (10 mg/kg), 28% (20 mg/kg), and 43% (40 mg/kg). During the acquisition trial, no differences in latent time are observed. Timosaponin AIII (20, 40 mg/kg, p.o.) potently inhibits this reduction of acetylcholine in scopolamine-treated mouse brain. The inhibitory effect of Timosaponin AIII is comparable to that of tacrine, which is used as a positive control.

Timosaponin AIII

Others

Other Targets

mTORC1

Others-Field

——

41059-79-4

740.92

C39H64O13

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (168.71 mM)

[C@]12([C@@H]3[C@H](C[C@H]1[C@H]1[C@H](CC2)[C@@]2([C@H](CC1)C[C@H](CC2)O[C@H]1[C@H](O[C@@H]2O[C@@H]([C@H]([C@@H]([C@H]2O)O)O)CO)[C@H]([C@H]([C@H](O1)CO)O)O)C)O[C@@]1([C@H]3C)CC[C@H](CO1)C)C

[(25S)-5?-Spirostan-3?-yl]2-O-(?-D-glucopyranosyl)-?-D-galactopyranoside

(-20℃)

With Ice Pack

≥ 2 years