DIM-C-pPhOH

Research use only, not for human use.

Nur77 (NR4A1) antagonist. Inhibits TGF-β induced cell migration of breast cancer cell lines. Promotes ROS/endoplasmic reticulum stress and proapoptotic pathways in pancreatic cancer cell lines.

DIM-C-pPhOH is a Nur77 Antagonist. DIM-C-pPhOH inhibits lung cancer cell and tumor growth in a metastasis model.(In Vitro):DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation.DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8.DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases.DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines.(In Vivo):DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth.

DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation.DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8.DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases.DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines. Cell Proliferation Assay Cell Line:ACHN and 786-O cells Concentration:7.5 μM, 15 μM, 20 μM Incubation Time:24 hours Result:Significantly decreased cell proliferation.Apoptosis Analysis Cell Line:ACHN and 786-O cells Concentration:20 μM Incubation Time:24 hours Result:Induced apoptosis in ACHN and 786-O cells.Western Blot Analysis Cell Line:ACHN and 786-O cells Concentration:15 μM, 20 μM Incubation Time:24 hours Result:Inhibited NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induced sestrin 2, activated AMPKα and inhibited activation of mTOR and downstream kinases.

DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth. Animal Model:Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells Dosage:30 mg/kg/day Administration:Oral gavage; daily; for 50 days Result:Resulted in a significant inhibition of tumor growth.

DIM-C-pPhOH

Others

Antioxidant

Nur77

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151358-47-3

338.41

C23H18N2O

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (369.39 mM)

Oc1ccc(cc1)C(c3cnc2ccccc23)c5cnc4ccccc45

1,1-Bis(3'-indolyl)-1-(p-hydroxyphenyl)methane

(-20℃)

With Ice Pack

≥ 2 years