D-Biotin

Research use only, not for human use.

Biotin is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

Biotin is a water-soluble B-vitamin and is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.(In Vitro):Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively.Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min.Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 μM)-induced cell death and reduces cell adhesion.(In Vivo):Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice.Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose.

Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with Kms of 9.24 μM and 53.1 μM, respecetively.Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with Vmax of 27.34 pmol/mg protein/min.Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50?μM)-induced cell death and reduces cell adhesion. Cell Viability Assay Cell Line:Murine oligodendrocyte 158N cells Concentration:1, 10, 100, 1000?nM Incubation Time:24 hours Result:Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status.Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.

Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice.Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose. Animal Model:Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)Dosage:15 mg/kg/d Administration:Oral gavage; 12 days Result:Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage.Animal Model:Grass carp (117±0.5 g)Dosage:0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg Administration:Oral gavage; 70 days Result:Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM).Reduced the mRNA levels of anti-microbial substances.Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.

D-Biotin | NSC 63865 | Vitamin B7

Metabolic Enzyme/Protease

Vitamin

VB7

——

——

58-85-5

244.31

C10H16N2O3S

>98% (HPLC)

DMSO: 49 mg/mL (200.56 mM)

C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2

5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid

(-20℃)

With Ice Pack

≥ 2 years