CASIN

Research use only, not for human use.

CASIN is a specific inhibitor of GTPase Cdc42 (IC50: 2 uM).

CASIN is an inhibitor of Cdc42 GTPase, a small GTPase of the Rho-subfamily that plays important roles in cytoskeleton organization, cell cycle progression, signal transduction, and vesicle trafficking. CASIN generated a rejuvenated phenotype of Hematopoietic stem cells (HSCs), reversing the aging-related and polarity phenotype of aged HSCs to that of young HSCs in vivo.

CASIN (0-10 μM; 3 d) inhibits the growth of AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells.CASIN (0-10 μM; 16 h) inhibits melphalan induced FANCD2 mono-ubiquitination in LR5 cells but shows no effect to S cells.CASIN (5 μM; 2 d) increases cell apoptosis of bortezomib-resistant MM patient cells.Cell Viability Assay Cell Line:AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells Concentration:0-10 μM Incubation Time:3 daysResult:Inhibited MM cell lines including AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells with GI50s vof 3.23, 4.31, 4.87, 2.56, 4.64, 4.67 and 3.97 μM, respectively.Western Blot Analysis Cell Line:MM (S) and melphalan-resistant (LR5) MM cells Concentration:5 μM Incubation Time:16 hours Result:Abolished melphalan induced FANCD2 mono-ubiquitination and DNA damage in LR5, sensitized LR5 to melphalan-induced cell death by blocking FANCD2-mediated DNA damage repair.Apoptosis Analysis Cell Line:Bortezomib-resistant MM patients cellsConcentration:5 μM Incubation Time:2 days Result:2-fold increased cell apoptosis in the cell derived from bortezomib-resistant MM patients.

CASIN (20 mg/kg; i.p. 2 times a day) prolongs lifetime of mice with busulfan injection. Animal Model:NSG mice with busulfan injectionDosage:20 mg/kg Administration:Intraperitoneal injection; 20 mg/kg 2 times a day Result:Significantly prolonged the lifespan of xenografts mice better than bortezomib, and showed a leading position when combined with bortezomib.

CASIN

Cell Cycle/DNA Damage

Integrin

STAT3

Cancer

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425399-05-9

306.41

C20H22N2O

>98% (HPLC)

DMSO : ≥ 31 mg/mL; 101.17 mM

C1CC(c2c(C1)c1c([nH]2)ccc(c1)c1ccccc1)NCCO

2-[(2,3,4,9-Tetrahydro-6-phenyl-1H-carbazol-1-yl)amino]ethanol

(-20℃)

With Ice Pack

≥ 2 years