Tizoxanide

Research use only, not for human use.

Tizoxanide is the main metabolite of nitazoxanide, which is a synthetic nitrothiazolyl-salicylamide derivative and an antiprotozoal agent.

Tizoxanide is the main metabolite of nitazoxanide, which is a synthetic nitrothiazolyl-salicylamide derivative and an antiprotozoal agent.Parasite Infection Approved(In Vitro):Tizoxanide (TIZ) induces mild mitochondrial uncoupling and activate AMPK in hepatocytes.Tizoxanide (TIZ) inhibits hepatitis C virus (HCV) replication in HCV replicon systems.(In Vivo):Tizoxanide (TIZ) (10 mg/kg; i.v.) can pass the BBB and distribute to the brain tissue. Tizoxanide retains in brain is obviously longer than that in plasma.

Tizoxanide (TIZ)induces mild mitochondrial uncoupling and activate AMPK in hepatocytes.Tizoxanide (TIZ)inhibits hepatitis C virus (HCV) replication in HCV replicon systems.Cell Viability Assay Cell Line:HepG2 cells Concentration:1-25 μmol/L Incubation Time:24 h Result:Induced mitochondrial uncoupling and AMPK activation not due to the non-specific cytotoxicity in HepG2 cells at concentrations less than 25 μmol/L. Immunofluorescence Cell Line:HepG2 cells Concentration:10 μmol/L Incubation Time:24 h Result:Promoted the nuclear translocation of TFEB by activating AMPK in HepG2 cells.RT-PCR Cell Line:HepG2 cells Concentration:1-25 μmol/L Incubation Time:24 h Result:Induced the increase transcription of SQSTM1/P62 in HepG2 cells.Western Blot Analysis Cell Line:HepG2 cells Concentration:1-25 μmol/L Incubation Time:24 h Result:Activated AMPK and increased phosphorylation of ACC in HepG2 cells.Cell Cytotoxicity Assay Cell Line:RP7 cells, NTZ-11 and TIZ-9 cell lines Concentration:The initial drug concentration was 0.02 μM, followed by 0.05, 0.1, 0.5, and 1 μM, and subsequent weekly increases of 1 μM until a final concentration of 11 μM.Incubation Time:3 days Result:Againsted HCV replication in RP7 cells (IC50: 0.16), NTZ-11 (IC50:1.2) and TIZ-9 cell lines (IC50: 1.5)

Tizoxanide (TIZ)(10 mg/kg; i.v.) can pass the BBB and distribute to the brain tissue. Tizoxanide retains in brain is obviously longer than that in plasma. Animal Model:Sprague Dawley rats Dosage:10 mg/kg Administration:Tizoxanide (10 mg/kg; i.v.)Result:Could pass the BBB and distribute to the brain tissue.

NSC697856 | Tizoxanide | Desacetylnitazoxanide | Desacetyl-nitazoxanide

GPCR/G Protein

Antibacterial

Antiviral

Infection

Parasite Infection

173903-47-4

265.2453

C10H7N3O4S

>98% (HPLC)

DMSO: ≥ 38 mg/mL

O=C(NC1=NC=C([N+]([O-])=O)S1)C2=CC=CC=C2O

Benzamide, 2-hydroxy-N-(5-nitro-2-thiazolyl)-

(-20℃)

With Ice Pack

≥ 2 years