Digiferruginol has cytotoxic activity. Digiferruginol and hederagenin show weak effect on porcine respiratory and reproductive syndrome virus (PRRSV) with an IC50 value of 80.5 ± 16.9 uM(SI = 19.9) and 43.2 ± 7.4 uM (SI = 13.1), respectively.

Palomid 529 has been used in trials studying the treatment of Age-Related Macular Degeneration.

TG2-IN-3h is a transglutaminase (TGase) inhibitor used in the study of neurological and immune system disorders.