CC214-1

Research use only, not for human use.

CC214-1 is an mTOR inhibitor with potential anticancer activity, inhibits protein translation, and induces autophagy.

CC214-1 is a potentially efficacious mTORinhibitor that induces autophagy ,with an IC50 is 0.002 μM. CC214-1 proved to be useful as an in vitro tool compound for the exploration of mTORkinase biology. CC214-1 can be used for Glioblastoma study.

Western Blot AnalysisCell Line:glioblastomaConcentration:0, 0.1, 1, 2, 5, 10 μM; 2 μM Incubation Time:8 h; 24 h Result:Inhibited mTORC1 signaling in all glioblastoma cell lines tested, potently suppressing rapamycin-resistant 4E-BP1 and mTORC2 signaling.Inhibited mTORC1-dependent 4E-BP1 and S6 phosphorylation in EGFRvIII-expressing glioblastoma cells, as well as blocked glioblastoma cells overexpressing wild-type EGFR.Immunofluorescence Cell Line:glioblastoma Concentration:2 μM, 5 μM Incubation Time:4 h; 0, 4, 12, 24, 48 h Result:Induced a transient expression of LC3B-II isoform and the conjugation of Atg12 to Atg5 indicative of the stimulation of the autophagy flux in U87EGFRvIII cell line. Massively lipidated LC3B-I to LC3B-II subtype and induces autophagy in GBM39 cells.Cell Cycle Analysis Cell Line:glioblastoma Concentration:0-10 μM Incubation Time:4 day Result:Induced TEE cells in CD4+ and CD8+ T cell subsets.

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Autophagy

Autophagy

Autophagy | mTOR

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1021920-32-0

391.43

C20H21N7O2

>98% (HPLC)

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O=C1NC2=NC=C(N=C2N1CCC3CCOCC3)C=4C=CC(=CC4)C5=NN=CN5

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(-20℃)

With Ice Pack

≥ 2 years