N-Aminofluorescein

Research use only, not for human use.

N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).

N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu2+. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu2+ in biological systems with λex/em=495/516 nm.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). N-Aminofluorescein consists of a fluorescein moiety and a hydrazide group to recognize and bind Cu2+, can promote the hydrolysis of amide.N-Aminofluorescein (FG) shows selectivity on Cu2+ and shows the absorption and emission bands at 632 nm and 515 nm in 70% aqueous HEPES buffered solution (pH 7.4) containing Cu2+.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). General procedure for Cu2+ determination:1.Prepare the 1.0 mM stock solution of spiro form fluorescein hydrazide in ethanol;Conduct the fluorescence-on reaction in 0.01 M Tris-HCl buffer (pH 7.2), with 10 μM N-Aminofluorescein;3.Add an appropriate volume of sample solution with a final Cu2+ concentration of not more than 10 μM, and adjust the final volume 10 mL with 0.01 M Tris-HCl buffer (pH 7.2);4.After 2 h, transfer a 3-mL portion of the solution to a 1-cm quartz cell, and measure the fluorescence intensity/spectrum at room temperature with λex/em = 495/516 nm and both excitation and emission slit widths of 5 nm;5.In the meantime, prepare a blank solution containing no Cu2+ and measure with the same conditions for comparison.

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Others

Other Targets

Others

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98907-26-7

346.34

C20H14N2O4

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (360.92 mM; Ultrasonic )

O=C1C=2C=CC=CC2C3(C4=CC=C(O)C=C4OC5=CC(O)=CC=C53)N1N

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(-20℃)

With Ice Pack

≥ 2 years