X-Gluc cyclohexanamine

CAS No. 114162-64-0

X-Gluc cyclohexanamine( —— )

Catalog No. M37185 CAS No. 114162-64-0

X-Gluc cyclohexanamine is a dye used to detect β-glucuronidase (GUS) activity and serves as a marker for gene expression, enabling the detection of target gene expression.

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
Size Price / USD Stock Quantity
1 mL x 10 mM in DMSO 28 In Stock
10MG 33 In Stock
25MG 65 In Stock
50MG 99 In Stock
100MG 152 In Stock
200MG Get Quote In Stock
500MG Get Quote In Stock
1G Get Quote In Stock

Biological Information

  • Product Name
    X-Gluc cyclohexanamine
  • Note
    Research use only, not for human use.
  • Brief Description
    X-Gluc cyclohexanamine is a dye used to detect β-glucuronidase (GUS) activity and serves as a marker for gene expression, enabling the detection of target gene expression.
  • Description
    X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
  • In Vitro
    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.3. Allow the plates to air dry and be used to inoculate the organisms.4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
  • In Vivo
    ——
  • Synonyms
    ——
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    Others
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    114162-64-0
  • Formula Weight
    521.79
  • Molecular Formula
    C20H26BrClN2O7
  • Purity
    >98% (HPLC)
  • Solubility
    ——
  • SMILES
    NC1CCCCC1.O[C@@H]1[C@@H](O)[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)O[C@@H]([C@H]1O)C(O)=O
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. ?
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