BODIPY-Cholesterol
CAS No. 878557-19-8
BODIPY-Cholesterol( —— )
Catalog No. M35243 CAS No. 878557-19-8
BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.
Purity : >98% (HPLC)
COA
Datasheet
HNMR
HPLC
MSDS
Handing Instructions
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Biological Information
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Product NameBODIPY-Cholesterol
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NoteResearch use only, not for human use.
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Brief DescriptionBODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.
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DescriptionBODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm).
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In VitroBODIPY-cholesterol assayTo prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM.Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration.Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 μM), mixed thoroughly and incubated for 10 -20 mins at 37 °C.The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment.
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In Vivo——
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Synonyms——
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PathwayOthers
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TargetOther Targets
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RecptorOthers
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Research Area——
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Indication——
Chemical Information
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CAS Number878557-19-8
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Formula Weight576.61
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Molecular FormulaC36H51BF2N2O
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Purity>98% (HPLC)
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SolubilityIn Vitro:?DMSO : 1 mg/mL (1.73 mM) H2O : < 0.1 mg/mL (insoluble)
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SMILES[H][C@@]1(CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCC1=C2C(C)=CC(C)=[N+]2[B-](F)(F)n2c(C)cc(C)c12
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Chemical Name——
Shipping & Storage Information
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Storage(-20℃)
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ShippingWith Ice Pack
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Stability≥ 2 years
Reference
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Cltrinin
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(R)-3-Hydroxybutanoi...
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