BNTA

Research use only, not for human use.

BNTA is a potent extracellular matrix (ECM) modulator. In a rat model of arthritis, BNTA modulates arthritis by promoting the synthesis of cartilage structural molecules on chondrocytes through the induction of superoxide dismutase 3 (SOD3) and regulating chondrogenesis through superoxide anion elimination.

BNTA, a potent extracellular matrix (ECM) modulator, facilitates cartilage structural molecule synthesis on chondrocytes by activating superoxide dismutase 3 (SOD3). BNTA shows a promising potential for osteoarthritis alleviation by modulating cartilage generation.

BNTA (0.01-10?μM; 1-7 d) does not decrease cell viability of human osteoarthritis chondrocytes and rat primary chondrocytes.BNTA (0.1 μM; 2 d) increases SOX9 protein markedly.BNTA (0.1 μM; 2 d) remarkably increases the COL2A1 and SOX9 protein levels in IL1β-induced rat OA chondrocytes.BNTA (10 μM; 5 d) increases proteoglycan staining in ATDC5 cells.BNTA (0.01-10?μM; 6 h) upregulates the expression levels of ECM-related genes COL2A1, ACAN, proteoglycan 4 (PRG4), and SRY-box 9 (SOX9) in human OA chondrocytes.BNTA (0.01-10?μM; 6 h) increases Col2a1, Acan, Prg4, and Sox9 mRNA levels, with maximum effects around 0.1?μM in IL1β-induced rat OA chondrocytes.BNTA (0.01-1?μM; 2 or 3 w) enhances anabolism and inhibited inflammatory response in osteoarthritis cartilage explants.Cell Viability Assay Cell Line:Human OA chondrocytes Concentration:0.01, 0.1, 1, 10 μM Incubation Time:1, 3, 5, 7 d Result:No toxicity was observed.Western Blot Analysis Cell Line:Human OA chondrocytes Concentration:0.1 μM Incubation Time:2 d Result:Elevated SOX9 protein compared with vehicle.

BNTA (0.015-1.5 mg/kg; intra-articular injection; twice a week for 4 and 8 weeks) could attenuate OA progression developed after anterior cruciate ligament transection (ACLT) in rats.Animal Model:Male SD rats weighing 80?g are induced by ACLT Dosage:0.015, 0.15, 1.5 mg/kg Administration:Intra-articular injection; twice a week for 4 and 8 weeks Result:Attenuated post-traumatic osteoarthritis development after intra-articular injection for 4 and 8 weeks and was well tolerated.

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Others

Other Targets

Reactive Oxygen Species

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685119-25-9

456.76

C17H11BrClNO3S2

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (218.93 mM; Ultrasonic )

Clc1ccccc1C(=O)Nc1c(Br)scc1S(=O)(=O)c1ccccc1

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(-20℃)

With Ice Pack

≥ 2 years