AM404

Research use only, not for human use.

AM404 is an endocannabinoid reuptake inhibitor, a TRPV(1) activator, a paracetamol metabolite, with neuroprotective and anticancer activity that blocks anandamide transport.

AM404, an inhibitor of endocannabinoid reuptake, blocks anandamide transport with IC50 values in the low micromolar range. AM404 is able to relax rat isolated hepatic arteries contracted with Phenylephrine, with a pEC50 value of 7.4 (corresponding to an EC50 of 0.04 μM). Neuroprotective Effect.

AM404 reduces C6 glioma cell proliferation with IC50 values of 4.9 μM. AM404 non-specifically inhibit C6 glioma cell proliferation at concentrations used to block the cellular accumulation of the endocannabinoid anandamide.Cell Viability Assay Cell Line:Rat C6 glioma cells Concentration:1, 3, 10 and 30 μM Incubation Time:24, 48, 72 and 96 h Result:Produced a concentration-dependent reduction in cell proliferation that was seen with 24 h of exposure to 10 and 30 μM concentrations and after 48 h at 3 μM. The lowest concentration of AM404 tested, 1 μM, produced a significant, albeit small, reduction in cell proliferation at 72 h.

AM404 (1-5?mg/kg, i.p.) exerts dose-dependent anxiolytic-like effects in the three models: elevated plus maze, defensive withdrawal and separation-induced ultrasonic vocalizations.Animal Model:Adult male Sprague-Dawley rats (250-300?g) Dosage:2.5-10?mg/kg Administration:Intraperitoneal (i.p.)Result:Caused a dose-dependent increase in anandamide levels in prefrontal cortex, hippocampus and thalamus.

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Membrane Transporter/Ion Channel

TRP/TRPV Channel

TRP/TRPV Channel | Cannabinoid Receptor

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183718-77-6

395.58

C26H37NO2

>98% (HPLC)

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N(C(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O)C1=CC=C(O)C=C1

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(-20℃)

With Ice Pack

≥ 2 years