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7-hydroxycoumarinyl-γ-Linolenate

CAS No. 161180-12-7

7-hydroxycoumarinyl-γ-Linolenate( —— )

Catalog No. M34987 CAS No. 161180-12-7

7-hydroxycoumarinyl-γ-Linolenate is a γ-linolenic acid ester of 7-hydroxycoumarin (umbelliferone) that behaves as a substrate for cPLA2. Hydrolysis of 7-hydroxycoumarinyl-γ-linolenate by phospholipase results in the release of the fluorescent compound 7-hydroxycoumarin which can be monitored spectrophotometrically (excitation at 335 nm, emission at 450 nm).

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
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Biological Information

  • Product Name
    7-hydroxycoumarinyl-γ-Linolenate
  • Note
    Research use only, not for human use.
  • Brief Description
    7-hydroxycoumarinyl-γ-Linolenate is a γ-linolenic acid ester of 7-hydroxycoumarin (umbelliferone) that behaves as a substrate for cPLA2. Hydrolysis of 7-hydroxycoumarinyl-γ-linolenate by phospholipase results in the release of the fluorescent compound 7-hydroxycoumarin which can be monitored spectrophotometrically (excitation at 335 nm, emission at 450 nm).
  • Description
    7-Hydroxycoumarinyl-γ-linolenate is a cytosolic phospholipase A2 (cPLA2) fluorogenic substrate. 7-Hydroxycoumarinyl-γ-linolenate can be used to monitor the enzymatic activity of cPLA2.
  • In Vitro
    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).cPLA2α activity assay:1. Sonicate 200μL of 1,2-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC)/Triton X-100 high-affinity mixed micelle solution containing 960μM Triton X-100 (HY-Y1883A), 60μM DTPC, 50μM 7-hydroxycoumarinyl-γ-linolenate, 50mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (pH?=7.2), 0.3mM EDTA (HY-Y0682), 1mM CaCl2 and 300mM KCl.2. Monitoring the hydrolysis of 7-hydroxycoumarinyl-γ-linolenate continuously with excitation at 360nm and emission at 460nm.3. The formation of 7-hydroxycoumarin was monitored by fluorospectrometer..
  • In Vivo
    ——
  • Synonyms
    ——
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    Others
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    161180-12-7
  • Formula Weight
    422.56
  • Molecular Formula
    C27H34O4
  • Purity
    >98% (HPLC)
  • Solubility
    ——
  • SMILES
    O(C(CCCC/C=C\C/C=C\C/C=C\CCCCC)=O)C=1C=C2C(=CC1)C=CC(=O)O2
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Xiao Q, et al. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep. 2016 Apr 29;6:25298.?
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