Ac-LETD-AFC

Research use only, not for human use.

Ac-LETD-AFC is a fluorescent substrate specifically cleaved by caspase-8 and can be used to quantify caspase activity by fluorescence detection of free AFC.Ac-LETD-AFC has an excitation wavelength of 400 nm and an emission wavelength of 505 nm.

Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Caspase activity assay:1. Incubate the cells according to your normal protocol.2. Wash cells once with PBS and centrifugate.3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL.4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol.5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer.

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Others

Other Targets

Others

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210345-02-1

729.65

C31H38F3N5O12

>98% (HPLC)

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C(F)(F)(F)C=1C=2C(=CC(NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC(C)C)NC(C)=O)=O)CCC(O)=O)=O)[C@@H](C)O)=O)CC(O)=O)=O)=CC2)OC(=O)C1

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(-20℃)

With Ice Pack

≥ 2 years