DFHO

Research use only, not for human use.

DFHO is a Corn homologous ligand that fluoresces yellow upon excitation and is used to image RNA in living cells.

DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.

Corn is tested in E. coli. Minimal fluorescence that is seen in DFHO-treated cells (20 μM) transformed with the empty vector. However robust yellow fluorescence is seen in E. coli expressing Corn.Corn-DFHO exhibits markedly enhanced photostability in vitro. The Corn-DFHO complexes are imaged in mammalian cells. Since the U6 promoter is one of the three major Pol III promoters, Corn from the U6 promoter is expressed and replaced the majority of the U6 sequence with Corn, leaving a minimal 5′ capping element leader sequence. The majority of the cells expressed diffuse yellow fluorescence, most prominently in the cytoplasm).DFHO exhibits low background fluorescence and cytotoxicity, making it a potentially suitable fluorophore for activation by RNA aptamers and for imaging experiments in living cells.

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Others

Other Targets

Others

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1420815-34-4

281.21

C12H9F2N3O3

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (355.61 mM; Ultrasonic )

O=C1C(N=C(C=NO)N1C)=CC2=CC(F)=C(O)C(F)=C2

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(-20℃)

With Ice Pack

≥ 2 years