4-Azido-L-phenylalanine

CAS No. 33173-53-4

4-Azido-L-phenylalanine( —— )

Catalog No. M33249 CAS No. 33173-53-4

4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments.

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
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Biological Information

  • Product Name
    4-Azido-L-phenylalanine
  • Note
    Research use only, not for human use.
  • Brief Description
    4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments.
  • Description
    4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
  • In Vitro
    In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions.
  • In Vivo
    ——
  • Synonyms
    ——
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    Others
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    33173-53-4
  • Formula Weight
    206.2
  • Molecular Formula
    C9H10N4O2
  • Purity
    >98% (HPLC)
  • Solubility
    In Vitro:?H2O : 25 mg/mL (121.24 mM; ultrasonic and adjust pH to 11 with NaOH)DMSO : 5 mg/mL (24.25 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 80°C )
  • SMILES
    N[C@@H](CC1=CC=C(C=C1)N=[N+]=[N-])C(O)=O
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Albayrak C, et al. Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation. Nucleic Acids Res. 2013 Jun;41(11):5949-63.?
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