TH1085

Research use only, not for human use.

TH1085 is an enhancer of OGG1. TH1085 stimulates DNA repair and protects cells from the environmental hazard paraquat (PQ).

TH1085 is an enhancer of OGG1. TH1085 stimulates DNA repair and protects cells from the environmental hazard paraquat (PQ).

TH10785 (6.25 μM, 30 min) induces a de novo β, δ-elimination in vitro, allowing for AP sites as new substrates.TH10785 (10 μM, 0-2 min) allows OGG1 to increase DNA repair by addressing AP sites.TH10785 (0-20 μM, 72 h) induces OGG1 β, δ-lyase activity shifts cells toward PNKP1 dependence.TH10785 (2 μM) has affinity to OGG1 (KD=5.5 μM) increased when adding an AP site analog containing double-stranded DNA (KD=1.3 μM). Cell Viability AssayCell Line:U2OS cellsConcentration:0-20 μM Incubation Time:72 hours Result:Reduced combination with PNKP1 inhibition for TH10785.Western Blot Analysis Cell Line:U2OS cells Concentration:0.65 μM; 10 μM Incubation Time:30 min; 24 h Result:Caused an up-regulation of members of the DDR through β, δ-elimination in combination with PNKP1 inhibition and APE1-independent de novo β, δ-elimination of AP sites by OGG1 in the presence of TH10785.RT-PCR Cell Line:U2OS cells Concentration:10 μM Incubation Time:1 h Result:Decreased oxidative damage in guanine-rich regions of the genome.Immunofluorescence Cell Line:U2OS OGG1-GFP cells Concentration:1 μM Incubation Time:0-2 min Result:Ecruited more OGG1 to laser-damaged sites.

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Others

Other Targets

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1002801-51-5

267.37

C17H21N3

>98% (HPLC)

In Vitro:?DMSO : ≥ 100 mg/mL (374.01 mM)

N=1C=2C=CC=CC2C(=NC1C3CC3)NC4CCCCC4

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(-20℃)

With Ice Pack

≥ 2 years