Sepin-1

Research use only, not for human use.

Sepin-1 is a noncompetitive inhibitor of separase with an IC50 of 14.8 μM. Sepin-1 can inhibit the growth of breast cancer xenograft tumors in mice and human cancer cell lines by inhibiting cell proliferation and inducing apoptosis.

Sepin-1 is a noncompetitive inhibitor of separase with an IC50 of 14.8 μM. Sepin-1 can inhibit the growth of breast cancer xenograft tumors in mice and human cancer cell lines by inhibiting cell proliferation and inducing apoptosis.

Cell Proliferation Assay Cell Line:BT-474, MCF7, MDA-MB-231, MDA-MB-468 cells Concentration:0-64 μM Incubation Time:3 days Result:Inhibited cell growth with EC50s of 18.03, 17.66, 27.33, 27.92 μM for BT-474, MCF7, MDA-MB-231, MDA-MB-468 cells, respectively.Western Blot Analysis Cell Line:BT-474, MCF7, MDA-MB-231, MDA-MB-468 cells Concentration:0, 10, 20, 40 μM Incubation Time:24 h Result:Decreased the expression of FoxM1 protein in a dose-dependent manner, and decreased teh expression of A-Raf, B-Raf, and C-Raf.

Animal Model:SCID beige mice (MCF7 xenografts)Dosage:10 mg/kg Administration:I.v.; daily for 5 days a week for 3 weeks Result:Inhibited tumor growth.

Sepin 1 | Sepin1 | 2H-Benzimidazole, 2,2-dimethyl-5-nitro-, 1,3-dioxide

Others

Other Targets

Endogenous Metabolite

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163126-81-6

223.188

C9H9N3O4

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (448.05 mM)

CC1(C)[N+]([O-])=c2ccc(cc2=[N+]1[O-])[N+]([O-])=O

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(-20℃)

With Ice Pack

≥ 2 years