N-Alpha-acetyllysine is a N-acetylated amino acid. It is a normal constituent of human urine with concentrations in normal samples too small to allow its routine detection; however it has been found at increased levels in the urine of a patient with aminoacylase I deficiency.

Acetamide is found in red beetrootis used primarily as a solvent and a plasticizer.

N-Acetyl-L-alanine is a substrate for Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit Myelin basic protein GTP-binding nuclear protein Ran Tropomyosin alpha 4 chain HIV-1 Rev binding protein 2 Xaa-Pro dipeptidase Thymosin beta-10 Actin-like protein 3 Alanine aminotransferase Serine/threonine protein phosphatase PP1-beta catalytic subunit 10 kDa heat shock protein (mitochondrial) Calmodulin and Beta-1-syntrophin.