Cyasterone

Research use only, not for human use.

Cyasterone, a natural EGFR inhibitor, can inhibit the growth of A549 and MGC823 cells, via regulating EGFR signaling pathway, it may be a promising anti-cancer agent.

Cyasterone was demonstrated potential inhibition effect in skin carcinoma cells.

Cyasterone exerts cytotoxicity on multiple cell lines: HeLa (IC50=77.24 μg/ml);HepG-2 (IC50=52.03 μg/ml);MCF-7 (IC50=82.07μg/ml) andMCF-7 (IC50=82.07 μg/ml). And It has none cytotoxicity (IC50>400 μg/ml) on 3 types of carcinoma (HT-29, Caco-2, T47D) and 1 type of normal (NIH 3T3) cell lines.Cyasterone (0-60 μg/ml; 48 hours) causes a significantly decreasing of the cell proliferation. It inhibits cell growth as a dose-dependent manner, exhibits IC50 values of 38.50 μg/ml and 32.96 μg/ml, respectively.Cyasterone (0-60 μg/ml; 24 hours) decreases p-EGFR, p-MEK, and p-mTOR as a dose-dependent manner in A549 cells, it affects the MAPK signaling pathway activities. Cell Viability Assay Cell Line:A549 cells and MGC823 cells Concentration:0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/mlIncubation Time:24 hours Result:Inhibited cellproliferation as a dose-dependent manner. Western Blot Analysis Cell Line:A549 cellsConcentration:0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml Incubation Time:24 hours Result:Surpressed p-EGFR, p-MEK, and p-mTOR expression.

Cyasterone (intraperitoneal injection; 5 mg/kg, 10 mg/kg and 15 mg/kg; 21 days) has anti-proliferation effect in vivo and inhibits MGC823 cells xenografted tumor growth in vivo with few changes in body weights. Animal Model:MGC823 xenograft tumor in BALB/C-nu mice Dosage:5 mg/kg, 10 mg/kg and 15 mg/kg Administration:Intraperitoneal injection Result:Inhibited MGC823 xenograft tumor growth in vivo.

Cyasterone | AI3-44890 | AI3 44890

Others

Other Targets

EGFR

Cancer

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17086-76-9

520.66

C29H44O8

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (192.07 mM)

O1C(=O)[C@H]([C@@H]([C@H]1C)C[C@H]([C@@](C)([C@H]1CC[C@]2(C3=CC(=O)[C@@H]4C[C@H]([C@H](C[C@@]4([C@H]3CC[C@]12C)C)O)O)O)O)O)C

5-beta-Stigmast-7-en-26-oic acid, 2-beta,3-beta,14,20,22,28-hexahydroxy-6-oxo-, gamma-lactone, (22R,24S,25S,28R)-

(-20℃)

With Ice Pack

≥ 2 years