Donepezil HCl

Research use only, not for human use.

Donepezil is a selective and effective AChE inhibitor for bAChE and hAChE (IC50: 8.12/11.6 nM).

Donepezil is a selective and effective AChE inhibitor for bAChE and hAChE (IC50: 8.12/11.6 nM).

Cell Viability Assay Cell Line:Cortical neuronal cellsConcentration:0.01, 0.1, 1, and 10 μM Incubation Time:24 hours Result:Exhibited significantly increased cell viability (maximized 89.2±2.1% in MTT, 96.3±5.5% in TBS, and 95.1±3.2% in CCK-8). Western Blot Analysis Cell Line:Cortical neuronal cells Concentration:10 μM Incubation Time:24 hours before 20 μM Aβ42 exposure for 6 hours Result:Effects of Donepezil on Akt and the GSK-3 signaling pathway were statistically significant in the presence of Aβ42 toxicity.

Animal Model:Male imprinting control region (ICR) mice (6 weeks old)Dosage:3-10 mg/kg Administration:Administered orallyResult:Pretreatment with 3–10 mg/kg ameliorated scopolamine-induced memory impairment. Animal Model:Hairless rats with an average weight of 300 g Dosage:3 and 10 mg/kg (Pharmacokinetic Analysis)Administration:Administered orally; and blood (250 μL) was collected through the tail vein Result:After oral treatment (3 and 10 mg/kg), a maximum concentration (Cmax) was reached after approximately 1.2 ± 0.4 h and 1.4 ± 0.5 h, respectively, and gradually decreased.

E2020

Others

Other Targets

bAChE| hAChE

Neurological Disease

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120011-70-3

416

C24H29NO3·HCl

>98% (HPLC)

H2O : 33.33 mg/mL 80.13 mM; DMSO : 6.2 mg/mL 14.91 mM

Cl.COc1c(OC)cc2C(=O)C(CC3CCN(Cc4ccccc4)CC3)Cc2c1

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(-20℃)

With Ice Pack

≥ 2 years