Methylisoindigotin

Research use only, not for human use.

Meisoindigo(Natura-α; N-Methylisoindigotin; Dian III), a derivative of Indigo naturalis, might induce apoptosis and myeloid differentiation of acute myeloid leukemia (AML).

Meisoindigo(Natura-α; N-Methylisoindigotin; Dian III), a derivative of Indigo naturalis, might induce apoptosis and myeloid differentiation of acute myeloid leukemia (AML).(In Vitro):Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines.Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia.Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest.Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells.Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells.(In Vivo):Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo.Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain.

Meisoindigo (Dian III; 5-20 μM; for 24?hours) inhibits growth of the AML cell lines. Meisoindigo (10?μM; for 24?hours) induces apoptosis of acute myeloid leukemia. Meisoindigo (5-10?μM; for 24?hours) causes cell-cycle arrest. Meisoindigo (5-10?μM; for 24?hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells. Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells. Cell Viability Assay Cell Line:HL-60, NB4, U937 leukemic cell lines Concentration:5, 10, 15, 20 μM Incubation Time:For 24?hours Result:Inhibited growth of the AML cell lines in a dose- and time-dependent manner.Apoptosis Analysis Cell Line:HL-60 cells Concentration:10?μM Incubation Time:For 24?hours Result:Induced apoptosis of acute myeloid leukemia.Cell Cycle Analysis Cell Line:HL-60 cells Concentration:5, 10?μM Incubation Time:For 24?hours Result:Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner.Western Blot Analysis Cell Line:HL-60 cells Concentration:5, 10?μM Incubation Time:For 24?hours Result:Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells.

Meisoindigo (Dian III; 50-150?mg/kg; IP; daily; for 14 days) has anti-leukemic activity in?vivo. Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain. Animal Model:NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cellsDosage:50, 100, 150?mg/kg Administration:IP; daily; for 14 days Result:Had anti-leukemic activity in?vivo.

Methylisoindigotin

Apoptosis

Apoptosis

Apoptosis inducer

Cancer

——

97207-47-1

276.29

C17H12N2O2

>98% (HPLC)

DMSO: 10 mM

CN(C1=CC=CC=C1/C2=C3C(NC4=C\3C=CC=C4)=O)C2=O

(E)-1,1'-dimethyl-[3,3'-biindolinylidene]-2,2'-dione

(-20℃)

With Ice Pack

≥ 2 years