NP-004255

Research use only, not for human use.

NP-004255 (NP004255, Corilagin) is a small molecule binds specifically to DNA repair protein RAD52 and disrupts the RAD52-ssDNA interaction, inhibits ssDNA binding with IC50 of 1.5 uM.

NP-004255 (NP004255, Corilagin) is a small molecule binds specifically to DNA repair protein RAD52 and disrupts the RAD52-ssDNA interaction, inhibits ssDNA binding with IC50 of 1.5 uM.(In Vitro):Corilagin (0-50 μM, 24 h) inhibits SGC7901 and BGC823 cell growth, and the cells became rounded.Corilagin (0-30 μM, 24 h) induces SGC7901 and BGC823 cell apoptosis.Corilagin (0-30 μM, 24 h) decreased the protein levels of procaspase-8, -9 and -3 and increases cleaved PARP in SGC7901 and BGC823 cell.Corilagin (0-30 μM, 24 h) induces autophagy in SGC7901 and BGC823 cell (enhancement of acidic vesicles, increased the level of LC3II).Corilagin (0-30 μM, 24 h) induces ROS generation in SGC7901 and BGC823 cell.Corilagin (40 μM, 24 or 48 h) induces G2 cell cycle arrest and apoptosis in Hey and SKOv3ip cells.Corilagin (0-80 μM, 1-3 days) inhibits the secretion of TGF-β1 in Hey, SKOv3ip and HO8910PM cells.(In Vivo):Corilagin (15 mg/kg, i.p., for 7 days) shows anti-tumor activity in Hep3B hepatocellular carcinoma.Corilagin (0-20 mg/kg, i.p.) prevents APAP-induced hepatotoxicity in mice.Corilagin (10 and 100 mg/kg, i.p.) ameliorates Bleomycin-induced pulmonary fibrosis in mice.

Cell Viability Assay Cell Line:SGC7901 and BGC823 cell Concentration:0, 10, 20, 30, 40 and 50 μM Incubation Time:24 h Result:Inhibited cell proliferation in a concentration-dependent manner.Western Blot Analysis Cell Line:SGC7901 and BGC823 cell Concentration:0, 10, 20, 30 μM Incubation Time:24 h Result:Decreased the protein levels of procaspase-8, -9 and -3 and increased the level of cleaved PARP.

Animal Model:Hep3B hepatocellular carcinoma mouse model.Dosage:15 mg/kg Administration:Intraperitoneal injection (i.p.)Result:Inhibited tumor growth without toxic effects. Animal Model:APAP-induced hepatotoxicity in mice Dosage:0, 1, 5, 10, 20 mg/kg Administration:Intraperitoneal injection (i.p.)Result:Decreased APAP-induced serum ALT level, hepatic myeloperoxidase (MPO) activity, cytokines (TNF-α, IL-1β, and IL-6) production, malondialdehyde (MDA) activity, and ERK/JNK MAPK and NF-κB protein expressions.

NP004255 | Corilagin

Cell Cycle/DNA Damage

DNA Repair Protein

Others

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23094-69-1

634.45

C27H22O18

>98% (HPLC)

Ethanol: SolubleMethanol: SolublePyridine: Soluble （ < 1 mg/ml refers to the product slightly soluble or insoluble )

C1C2C(C(C(C(O2)OC(=O)C3=CC(=C(C(=C3)O)O)O)O)OC(=O)C4=CC(=C(C(=C4C5=C(C(=C(C=C5C(=O)O1)O)O)O)O)O)O)O

Glucopyranose, cyclic 3,6-(4,4',5,5',6,6'-hexahydroxydiphenate) 1-gallate, β-D- (8CI)

(-20℃)

With Ice Pack

≥ 2 years