LCS3

Research use only, not for human use.

LCS3 is a reversible and non-competitive synergistic inhibitor of glutathione disulfide reductase (GSR) and thioredoxin reductase 1 (TXNRD1) with IC50s of 3.3 μM and 3.8 μM, respectively.

LCS3 is a reversible and uncompetitive glutathione disulfide reductase (GSR) and thioredoxin reductase 1 (TXNRD1) inhibitor (IC50=3.3 μM and 3.8 μM, respectively). LCS3 shows anti-tumor activity, and induces apoptosis. LCS3 can be used in lung adenocarcinoma (LUAD) research.

LCS3 (5 nM-10 μM; 96 h) inhibits lung cancer cell lines, but not non-transformed lung cells.LCS3 (3 μM; 3, 6, and 12 h) induces ROS and NRF2 pathway activation in sensitive lung adenocarcinoma (LUAD) cells.LCS3 (3 μM; 96 h) selectively kills lung adenocarcinoma (LUAD) cell lines, in part through the induction of apoptosis.Cell Viability Assay Cell Line:Non-small cell lung cancer (NSCLC) cells and non-transformed lung cells Concentration:5 nM-10 μM Incubation Time:96 hours Result:Inhibited the growth of 24/25 NSCLC cell lines at low micromolar concentrations (IC50<5 μM), both of the non-transformed lung cell lines were relatively insensitive (IC50>10 μM).Cell Viability Assay Cell Line:H23 and H1650 cells Concentration:3 μM Incubation Time:3, 6, and 12 hours Result:Responded to LCS3 by accumulating ROS and activating the NRF2 transcription program.Apoptosis Analysis Cell Line:lung adenocarcinoma (LUAD) cells Concentration:3 μM Incubation Time:96 hours Result:Increased cleavage of caspase 3, caspase 7 and/or PARP1 in all LCS3-sensitive LUAD cell lines.Western Blot Analysis Cell Line:H23 and H1650 cells Concentration:3 μM Incubation Time:24 hours Result:Increased the protein levels of NRF2 and of the products of selected downstream targets of NRF2 in both cell lines.

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Apoptosis

Apoptosis

Apoptosis | Glutathione reductase

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109844-92-0

266.64

C11H7ClN2O4

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (468.80 mM; Ultrasonic (<60°C)

[O-][N+](=O)C1=CC=C(O1)C(=O)NC1=CC=C(Cl)C=C1

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(-20℃)

With Ice Pack

≥ 2 years