L82-G17

Research use only, not for human use.

L82-G17 is an uncompetitive LigI-selective inhibitor in human cells with utility as a probe of the catalytic activity and cellular functions of LigI.

L82-G17 is an uncompetitive LigI-selective inhibitor in human cells with utility as a probe of the catalytic activity and cellular functions of LigI.

L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding.L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation.L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage. Cell Viability Assay Cell Line:HeLa cells Concentration:0-30 μM Incubation Time:5 days Result:Reduced cell number by about 70% at 20 μM.Cell Proliferation Assay Cell Line:CH12F3 WT and CH12F3Δ/Δ cells Concentration:0-100 μM Incubation Time:72 h Result:Had great effect on the proliferation and survival of the parental CH12F3 cells.

——

——

Cell Cycle/DNA Damage

DNA/RNA Synthesis

FGFR2

——

——

92285-87-5

264.67

C11H9ClN4O2

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (472.29 mM)

OC1=CC=CC(\C=N\NC2=C(Cl)C(=O)NN=C2)=C1

——

(-20℃)

With Ice Pack

≥ 2 years