BODIPY-Cholesterol

Research use only, not for human use.

BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm).

BODIPY-cholesterol assayTo prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM.Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration.Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 μM), mixed thoroughly and incubated for 10 -20 mins at 37 °C.The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment.

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878557-19-8

576.61

C36H51BF2N2O

>98% (HPLC)

In Vitro:?DMSO : 1 mg/mL (1.73 mM) H2O : < 0.1 mg/mL (insoluble)

[H][C@@]1(CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCC1=C2C(C)=CC(C)=[N+]2[B-](F)(F)n2c(C)cc(C)c12

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(-20℃)

With Ice Pack

≥ 2 years