6RK73

Research use only, not for human use.

6RK73 is a covalent irreversible and specific UCHL1 inhibitor (IC50: 0.23 μM).

6RK73 is a covalent irreversible and specific UCHL1 inhibitor (IC50: 0.23 μM). 6RK73 shows almost no inhibition of UCHL3 (IC50: 236 μM).(In Vitro):6RK73 (5 μM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells. 6RK73 (5 μM; 24-48 hours) results in migration significantly slower than the DMSO control group in MDA-MB-436 cells.(In Vivo):6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish.

Cell Viability Assay Cell Line:MDA-MB-436 cells Concentration:5 μM Incubation Time:24, 48 hours Result:Migrated significantly slower than the DMSO control group Western Blot Analysis Cell Line:MDA-MB-436 cells Concentration: 5 μM Incubation Time:1, 2, 3 hours Result:Displayed strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels.

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Others

Other Targets

Antioxidant

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1895050-66-4

307.37

C13H17N5O2S

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (406.68 mM)

O=C(Nc1ncc(s1)N1CCOCC1)[C@H]1CCN(C1)C#N

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(-20℃)

With Ice Pack

≥ 2 years