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5-IAF

CAS No. 63368-54-7

5-IAF( —— )

Catalog No. M33250 CAS No. 63368-54-7

5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains).

Purity : >98% (HPLC)

COA Datasheet HNMR HPLC MSDS Handing Instructions
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Biological Information

  • Product Name
    5-IAF
  • Note
    Research use only, not for human use.
  • Brief Description
    5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains).
  • Description
    5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).
  • In Vitro
    8 μM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 μM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 μM 5-IAF.
  • In Vivo
    ——
  • Synonyms
    ——
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    Others
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    63368-54-7
  • Formula Weight
    515.25
  • Molecular Formula
    C22H14INO6
  • Purity
    >98% (HPLC)
  • Solubility
    In Vitro:?DMSO : 50 mg/mL (97.04 mM; Ultrasonic )
  • SMILES
    Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(NC(=O)CI)ccc23)c1
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374.?
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