A77-01

Research use only, not for human use.

A potent inhibitor of TGF-β type I receptor superfamily activin-like kinase ALK5 with IC50 of 25 nM.

A potent inhibitor of TGF-β type I receptor superfamily activin-like kinase ALK5 with IC50 of 25 nM; A-77-01 is a close analogue of A-83-01(Axon 1421) and has a very similar biological profile of A-83-01.

A-77-01 (0.01-10 μM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion.A-77-01 (1 μM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β.A-77-01 (1 μM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2. RT-PCR Cell Line:Mv1Lu cells Concentration:0.01 μM, 0.03 μM, 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM Incubation Time:25 hours Result:Inhibited the luciferase activity induced by TGF-β in concentration-dependent fashion.Western Blot Analysis Cell Line:HaCaT cells Concentration:1 μM Incubation Time:30 minutes Result:Inhibited TGF-β-induced phosphorylation of Smad2.

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A77-01 | A-77-01

TGF-beta/Smad

TGFBR

ALK5

Cancer

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607737-87-1

286.3306

C18H14N4

>98% (HPLC)

10 mM in DMSO

CC1=CC=CC(=N1)C2=C(C=NN2)C3=CC=NC4=CC=CC=C34

Quinoline, 4-[3-(6-methyl-2-pyridinyl)-1H-pyrazol-4-yl]-

(-20℃)

With Ice Pack

≥ 2 years